Title:
Investigation of Activated Blood Substances in the Measuring of Whole Blood Clotting Time During Hemodialysis
Abe K.
1 ;Abe K.
1 ;Abe K.
1 ;Abe K.
2 ;Abe K.
1
1. Hemodialysis Unit
; 2. Department of Blood Purification
E-mail address corresponding author:
tshibamoto.kidc@med.tmd.ac.jp
Background:
Using several whole blood activated partial thromboplastin time reagents used generically for monitoring whole blood clotting time during hemodialysis, we investigated the optimal activated blood substance to be used for measuring.
Methods:
Subjects included 86 end stage renal disease patients (ESRD) of whom 38 were using unfractionated heparin (UH), 35 were using low molecular weight heparin (LH), and 13 were using a protease inhibitor (PI). Four types of activated blood substances were examined. These were Pathromtin SL (SL), Actin Activated Chphaloplastin (AC), Cephotest (CT), and half-diluted CT (HCT). Method 1: Whole blood clotting time was measured using the four types of activated blood substances during hemodialysis, using UH and LH as the anticoagulants. Pathromtin (PR) containing kaolin was used on the subjects. Method 2: The whole blood clotting time was measured using the activated blood substance evaluated as optimal in method 1 during hemodialysis using PI as the anticoagulant. Celite was used on the subjects.
Results:
The whole blood clotting time in ESRD differed according to the active blood substance. Before and after anticoagulant administration, SL showed a higher value than DF, CT, and HCT. The activated substance CT was positively correlated with PR significantly, and HCT showed the highest correlation. The measurement of whole blood clotting time by HCT during PI use was seen to be positively correlated with Celite.
Conclusion:
The whole blood clotting time in chronic dialysis patients differed according to the activated blood substance used for measuring. Regardless of administration of an anticoagulant, HCT showed a significant positive correlation with the targeted active substances. HCT was the optimal activated blood substance during whole blood clotting time, and was useful as a unified method of monitoring anti-clotting time during hemodialysis.
Subject:
Hemodialysis
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